EMSA-Related

More and more researchers are using non-radioactive  techniques for DNA/RNA or protein detection. Non-radioactive detection primarily is depended on application of chemiluminescence catalyzed by HRP, AKP or other enzymes. The development of enhanced chemiluminescence (ECL) increases greatly the sensitivity of detection, and the sensitivity of chemiluminescence is considerably higher than that using radioisotopes for detection of DNA (Southern blotting), RNA (Northern blotting) and proteins (Western Blotting).

For example, using chemiluminescence, obtaining images usually takes only a few minutes, whereas obtaining autoradiographs with radioisotope-labeled molecules takes 16-48 hours.

Advantages for ECL-based detection:

High Sensitivity

Safer than using radioisotopes

Easy to handle

Much shorter time to obtain results

Long life for Biotin- or fluorescein-probes

In preparation of DNA/RNA-based non-radioactive probes, Biotin, DIG and fluorescein are used most commonly. Among these labeling molecules, Biotin is most widely used for its chemical stability, high affinity, fast binding time, small molecular weight and compact structure. The major technology developed by Viagene Biotech is high sensitive non-radioactive detection based on HRP-induced chemiluminescence. We have provided services for our customers in life sciences with non-radioactive detection kits since 2006. We have hundreds of premade biotin-labeled probes for EMSA and have years of experience for custom-synthesizing non-radioactive probes using either biotin or fluorescein labeling.