General EMSA kits

A. General EMSA kits and prices:

EMSA (electrophoretic mobility shift assay, mobility shift or gel shift) is a classic technology for detecting activated nucleic acid-binding proteins, most of which are transcriptional factors).

General non-radioactive EMSA kits don't include 1) nucleic acid probes and 2) nucleic acid-binding membranes. For using these kits, customers need to prepare biotin- or IR Fluo-labeled nucleic acid probes. Viagene provides 4 types of general EMSA Kits: 1) DNA EMSA using biotin-probes, 2) RNA EMSA using biotin-probes, 3) DNA EMSA using IR Fluo-probes, 4) RNA EMSA using IR Fluo-probes.

The kits using biotin-probes are similar to Light Shift EMSA of Thermo Fisher, but costs are much less. Our kits doesn't include EBNA (Epstein-Barr Nuclear Antigen) as controls because we think the EBNA control is unnecessary for regular EMSA (detail).

For RNA EMSA, IR EMSA RNA kit (SIDET202) is highly recommended because its simple operation and fast results, which would reduce the decay of RNA probe.  
 

B. Features of general EMSA kits:

(1) Non-radioactive EMAS.
(2) Long storage. Keeping kits at proper condition, they can keep >1 year, ready to use at any time.
(3) Operation is very simple and all steps after gel-electrophoresis are omitted.
(4) Fast results, 2-5 hours.
(5) Including Poly[dI:dC] for reducing non-specific interaction of nucleic acid and proteins.
(6) Suitable for variety of nucleic acid-protein interaction.
(7) Including several reagents for optimizing the interaction of nucleic acid and proteins.

C. General EMSA kits and their components: