RNA EMSA

  
        
RNA EMSA样图

In recent years, evidence has emerged for the existence of many diverse types of RNA, which play roles in a wide range of biological processes in all kingdoms of life. These molecules generally do not, however, act in isolation, and identifying which proteins partner with RNA is a major challenge. Many methods, in vivo and in vitro, have been used to address this question, including combinatorial or high-throughput approaches, such as systematic evolution of ligands, cross-linking and immunoprecipitation and RNA immunoprecipitation combined with deep sequencing. However, most of these methods are not trivial to pursue and often require substantial optimization before results can be achieved. Here, we demonstrate a simple technique that allows one to screen proteins for RNA-binding properties in a gel-shift experiment and can be easily implemented in any laboratory. This assay should be a useful first-pass tool for assessing whether a protein has RNA- or DNA-binding properties, prior to committing resources to more complex procedures.

The principle of RNA EMSA as well as DNA EMSA is simple, considerations for performing a successful RNA EMA including the follows:

1)Needs instruments and reagents for RNA EMSA.

2) The knowledge for handling RNAs and proteins.

3) The skills for running a vertical gel electrophoresis.

4) The facility for imaging ECL signals via x-ray films or imagers for imaging ECL or infrared fluorescence.

5) The RNA probe is expensive, 20 fold of what for making a DNA probes.

6) Most probes for RNA EMSA is a single-strand RNA, which is very fragile for degradation.

7) Working environment should keep as RNase-free.

8) Based on the considerations above, we recommend our IRFluo rEMSA kits for RNA EMSA for its fast results (~2 hours) and simple operation (imaging gels immediately after gel electrophoresis).

9) If using Viagene's RNA EMSA service, the results would be secured as the scientists in Viagene Biotech have extensive experience for RNA EMSA (Linking to technique services).

Related Products

Catalog# Products Description Pack Price($)
SIDET101 Coolshift-BId General ECL EMSA kit, DNA 100assays 277
SIDET102 Coolshift-BIr General ECL EMSA kit, RNA 100assays 399
SIDET201 Coolshift-IRd General IR EMSA kit, DNA 100assays 199
SIDET202 Coolshift-IRr General IR EMSA kit, RNA 100assays 298
BITFxxx detail Complete ECL EMSA kits 30/60assays 287/492
IRTFxxx detail Complete IR EMSA kits 30/60assays 287/492
IRTFxxx detail Gel-staining EMSA kits 50/100assays 129/198
SERV005a1 EMSA-PN Ctrl EMSA/positive+negatives 5 loads/ea. 95
SERV005a2 EMSA-PN Ctrl EMSA/positive+negatives 10 loads/ea. 155
SERV005b1 EMSA-P Ctrl EMSA/positive controls 5 loads/ea. 75
SERV005b2 EMSA-P Ctrl EMSA/positive controls 10 loads/ea. 119
SERV005c1 EMSA-N Ctrl EMSA/negative controls 5 loads/ea. 45
SERV005c2 EMSA-N Ctrl EMSA/negative controls 10 loads/ea. 59
TFRGT105a Poly[dI:dC] EMSA/non-specific inhibitor 50assays 64
TFRGT105b Poly[dI:dC] EMSA/non-specific inhibitor 100assys 99
TFRGT108a EMSA-Mmb EMSA/probe-membranes 5 sheets 55
TFRGT108b EMSA-Mmb EMSA/probe-membranes 10 sheets 99
TFRGT104a EMSA-RBu EMSA/reaction buffer 50assays 25
TFRGT104b EMSA-RBu EMSA/reaction buffer 100assays 35
TFRGT110a ESA-HRPb Streptavidin-HRP 50assays 65
TFRGT110b ESA-HRPb Streptavidin-HRP 100assys 99
TFRGT103a EMSA-BBuf ECL EMSA/blocking buf. 60ml/btl 45
TFRGT103b EMSA-WBuf ECL  EMSA/washing buf. 60ml/btl 59
TFRGT103c EMSA-EBuf ECL  EMSA/equilibration buf. 60ml/btl 21
TFxxxBP details Biotin-probes for EMSA 50assays 99
TFxxxBP details Biotin-probes for EMSA 100assys 159
TFxxxIR details IR Fluo-probes for EMSA 50assys 135
TFxxxIR details IR Fluo-probes for EMSA 100assys 199
TFxxxCP details Unlabeled competitive probes 6assays 45
TFxxxCP details Unlabeled competitive probes 12assays 65
TFxxxMP details Unlabeled mutant probes 6assays 59
TFxxxMP details Unlabeled mutant probes 12assays 89
SINP001a C-N extractn Cell Nuclear Extraction 50samples 99
SINP001b T-N extractn Tissue Nuclear Extraction 50samples 125
SINP013 EMSA-ProC Nucl. protein concentration 100mL/botl 85

  

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