What needs/EMSA

Performing a Successful EMSA needs contain lab devices (detail), and knowledge (detail). In the form below we list devices for performing EMSA for those who would like to carry out EMSA in lab. As Viagene's product line include almost all EMSA kits, reagents and devices, customers would find proper products from Viagene Biotech. If you have any question, please contact us. Furthermore, we provide any kind of EMSA and EMSA-related services ( check).

A. Reagents & Devices needed for non-radioactive EMSA

	Gel-stain EMSA	IR Fluo-EMSA	ECL/biotin EMSA
Sample prep. kits	Cat.#: SINP001a/b	Cat.#: SINP001a/b	Cat.#: SINP001a/b
protein concentr. kit	Cat.#: SINP013	Cat.#: SINP013	Cat.#: SINP013
Complete kits	Cat.#: GSTFxxx	Cat.#: IRTFxxx	Cat.#: BITFxxx
General kits	Cat.#: SIDET301	Cat.#: SIDET101	Cat.#:SIDET201
Probes	Non-labeled probes	IR Fluo-probes	Biotin-probes
Devices needed	Tissue culture hood Ultra-low freezer Refrigerator Water bath Spectrophotometer High-speed, cool centrifuge Lab shaker Electrophoresis devices   - - High sensitive 490nm imager/scanner	Tissue culture hood Ultra-low freezer Refrigerator Water bath Spectrophotometer High-speed, cool centrifuge Lab shaker Electrophoresis devices - - Near infrared fluorescence imagers/scanners	Tissue culture hood Ultra-low freezer Refrigerator Water bath Spectrophotometer High-speed, cool centrifuge Lab shaker Electrophoresis devices & trans-blot devices UV crosslinker Dark room & film developer or ECL imagers or scanner. High sensitive ECL imager/scanners
Cell/tissue-culture chemicals	Cell/tissue culture-related chemicals	Cell/tissue culture related chemicals	Cell/tissue culture-related chemicals

B. The devices used by Viagene for performing EMSA services and development of EMSA products.

Nuclear protein extraction kit: (SINP001, Viagene Biotech Biotech, Tampa, USA)

Nuclear protein concentration kit (CHEM001, Viagene Biotech, Tampa, USA)

General EMSA kits (check, Viagene Biotech, Tampa, US)

Complete EMSA kits with probes (check, Viagene Biotech, Tampa, USA)

Gel-stain EMSA kit (detail) (Viagene Biotech, Tampa, USA)

Biotin-labeled probes (check) (Viagene Biotech, Tampa, USA)

IR Fluo-probes (detail, Viagene Biotech Biotech, Tampa, USA)

Pipettors; 0-1.5ul,1-10ul,0-20ul,0-200ul,0-1000ul (Eppendorf's products)

Dual power supply (PSLB001, Viagene Biotech, Tampa, USA)

Bio-Rad trans-blot (Bio-Rad, Mini Trans-Blot, USA)

Semi-dry trans-blot (Bio-Rad, Semi Trans-Blot SD, USA)

Cooled centrifuge (Eppendorf, 5810R)

Cooled centrifuge (Eppendorf 5704R)

UV Crosslinker (STRATGEN UV Stratalinker 1800, USA)

Mini shaker (SIPX002 Viagene Biotech, Tampa, USA)

Chemiluminescence Imager (CoolImager III, IMGR002, Viagene Biotech, Tampa, USA)

Ultra-low freezer (-86C, Thermo Scientific, USA)

Lab refrigerator(s)

*If UV crosslinker is not available, other lab UV lights/sources may be used for crosslink of nucleic acid to membranes. However, as efficiency of crosslinking may be very low and the results are not consistent.