EMSA Troubleshooting
| Methods | Problems/Solution | Major procedures | Viagene's products |
|---|---|---|---|
| Pre-EMSA | Well design is a good start | Experimental design | Help customers for their studies |
| What needs for EMSA | Equipments & reagents | Devices, kit & reagents | |
| Bad samples cause major EMSA problems | Sample preparation |
Nuclear protein prep. Nuc. pro. concentration Positive controls. Sample Prep. service |
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| High quality probes are nescessary for EMSA | Probe preparation |
Ready biotin-probes Ready IR Fluo-probes Custom EMSA probes |
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Biotin/ECL EMSA |
See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
| ECL EMSA-related problems and their solution. |
Protein-probe reaction Running gels Transblots UV crosslink Biotin/Avidin/HRP reaction ECL Imaging |
Complete EMSA kits General EMSA kits EMSA reagents ECL substrates ECL imagers EMSA controls EMSA Services |
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IR
Fluo- EMSA |
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| See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
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| IR EMSA-related problems and their solution. |
Protein-probe reaction Running gels Imaging gels |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents EMSA controls EMSA Services |
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Gel-Stain EMSA |
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| See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
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| Gel-stain EMSA-related problems and their solution. |
Protein-probe reaction Running gels Staining gels Imaging gels |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services |
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Competitive EMSA |
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| See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
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| Competitive EMSA-related problems and their solution. |
Protein-probe reaction Running gels Staining gels Imaging gels |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services |
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Supershift EMSA |
See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
| Supershift EMSA-related problems and their solution. |
Protein-probe reaction The rest is same as ECL or IR EMSA |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services |
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| RNA EMSA | |||
| See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
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| RNA EMSA-related problems and their solution. |
Protein-probe reaction The rest is same as ECL or IR EMSA |
Custom probes General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Service |
